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PEI 聚乙烯亚胺,(MW 40,000),更高效的PEI聚乙烯亚胺聚合物转染试剂,24765-1
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  • 货号 24765-1
  • 品牌 Polysciences
  • CAS号 100%原装正品
  • 规格/包装 1g
  • 售卖单位
  • 储存条件
  • 现货状态 三个工作日

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北京明阳科华生物科技有限公司提供100%原装正品,

更高效的
PEI聚乙烯亚胺聚合物转染试剂

Polyethylenimine HCl MAX, Linear, Mw 40,000

线性化聚乙烯亚胺盐酸盐 MAXPEI 40000

基本描述:

Polyethylenimine HCl MAX, Linear, Mw 40,000PEI 40000)是热卖产品线性化聚乙烯亚胺PEI25,000的升级产品,不同之处在于1)完全水解(去乙酰化);2PEI25000的盐酸盐形式,具更高的水溶特性;3)含更长连续性乙烯亚胺(ethyleneimine)片段,其质子化氮水平比PEI25000提高11%以上。本品经碱中和后,可转化为线性化自由胺(PEI),此时分子量正相当于25,000

Thomas M,et al(2005)研究数据表明,去乙酰化的PEI 40,000(Polyethylenimine MAX 40,000)体外质粒DNA转染效率提高21倍,小鼠体内靶向效率达到10,000倍,随之肺部特异疗效显示增强1500倍。


PEI MAX 40K (also known as PEI 22K in free base) is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain an edge in their critical work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

PEI MAX 40K is easier to use and offers consistently higher titers than PEI 25K. PEI 25K transfection solutions typically take several hours to prepare, while PEI MAX 40K can be converted to a ready-to-use solution in under two hours. Additionally, PEI 25K contains 4-11% residual propionyl groups, which prevents the polymer backbone from strongly binding to DNA. PEI MAX 40K’s fully depropionylated structure means each batch performs consistently higher.


Comparison of PEI 25K (#23966) and PEI MAX 40K (#24765). 
Method: 10e6/mL HEK293 cells in 50 mL FS transfected with IgG64 plasmid pair. PEI:DNA 4:1. Samples taken 120 hpt. Quantified with Thermo Fisher #23310. N=4 each. Error bar = standard deviation.

A pre-made sterile-filtered solution of PEI MAX 40K is available as our Transporter 5™ Transfection Reagent.

CAS#: 
49553-93-7
Molecular Weight: 
40,000 (~22,000 free base)
Soluble In: 
Cold and room temperature water 
Insoluble in: Common organic solvents (ethanol, acetone, tetrahydrofuran)
Appearance: 
White to off-white free flowing solid
Reference(s):

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

** Delafosse, L., Xu, P. & Durocher, Y. Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells. Journal of Biotechnology 227, 103–111 (2016). doi:10.1016/j.jbiotec.2016.04.028

** Gutiérrez-Granados, S., Cervera, L., Segura, M. de las M., Wölfel, J. & Gòdia, F. Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells. Applied Microbiology and Biotechnology 100, 3935–3947 (2016). doi:10.1007/s00253-015-7213-x

Kobayashi, S., Yoshii, K., Hirano, M., Muto, M. & Kariwa, H. A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cellsJournal of Virological Methods 240, 14–20 (2017). doi:10.1016/j.jviromet.2016.11.006

** Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.

Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lungProc Natl Acad Sci U S A. 102(16):5679-84.

** Contains particularly useful information.

TSCA 

Hazards: 
Irritant
Handling: 
Glove, chemical goggles & fume hood
Storage: 
Store at room temperature
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